Testing Data #1
(Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human nestin (MBS224038))
Rabbit NESTIN Polyclonal Antibody | anti-Nes antibody
RABBIT ANTI HUMAN NESTIN
Gene Names
Nes; C78523; ESTM46; AA166324
Reactivity
Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
NESTIN; Polyclonal Antibody; RABBIT ANTI HUMAN NESTIN; anti-Nes antibody
Host
Rabbit
Reactivity
Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises human Nestin, an intermediate filament involved in cytoskeletal organisation.
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.
Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
This item has been reported to work in Immunofluorescence.
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.
Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
This item has been reported to work in Immunofluorescence.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Sequence Length
1864
Applicable Applications for anti-Nes antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 1/20000; Maximum Dilution: 1/80000;
Immunohistology - Paraffin: Maximum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/3000
Immunohistology - Paraffin: Maximum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/3000
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human Nestin (aa1484-1500).
Histology Positive Control Tissue
Histology Positive Control Tissue
Antiserum Preparation
Antisera to Human Nestin were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human nestin (MBS224038))
Testing Data #1
(Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human nestin (MBS224038))
Testing Data #2
(Staining of formalin fixed, paraffin embedded mouse kidney with Rabbit anti Human nestin (MBS224038))
Testing Data #2
(Staining of formalin fixed, paraffin embedded mouse kidney with Rabbit anti Human nestin (MBS224038))
Related Product Information for anti-Nes antibody
MBS224038 recognises human Nestin, an intermediate filament involved in cytoskeletal organisation. Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages. Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. MBS224038 has been reported to work in Immunofluorescence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
201,770 Da
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
Nes
NCBI Official Synonym Symbols
C78523; ESTM46; AA166324
NCBI Protein Information
nestin; intermediate filament protein
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
Nes
UniProt Entry Name
NEST_MOUSE
Uniprot Description
nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Protein type: Cytoskeletal
Cellular Component: cytoplasm; intermediate filament; intracellular
Molecular Function: protein binding; structural molecule activity; CCR5 chemokine receptor binding
Biological Process: nervous system development; multicellular organismal development; cell projection morphogenesis; negative regulation of neuron apoptosis
Research Articles on Nes
Similar Products
Product Notes
The Nes nes (Catalog #AAA224038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI HUMAN NESTIN reacts with Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NESTIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB). ELISA: Minimum Dilution: 1/20000; Maximum Dilution: 1/80000; Immunohistology - Paraffin: Maximum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/3000. Researchers should empirically determine the suitability of the Nes nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NESTIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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