Western Blot (WB)
(The anti-NEK9 Pab is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).)
Rabbit anti-Human, Mouse NEK9 Polyclonal Antibody | anti-NEK9 antibody
NEK9, CT (NIMA (Never in Mitosis Gene A)-Related Kinase 9, Never in Mitosis A-related Kinase 9, NIMA-related Kinase Nek9, NimA-related Protein Kinase 9, DKFZp434D0935, HGNC:18591, KIAA1995, MGC138306, MGC16714, Nercc1 Kinase, NERCC1, NERCC, NIMA-related K
Gene Names
NEK9; Nek8; NERCC; NERCC1; MGC16714; MGC138306; DKFZp434D0935
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
NEK9; Polyclonal Antibody; CT (NIMA (Never in Mitosis Gene A)-Related Kinase 9; Never in Mitosis A-related Kinase 9; NIMA-related Kinase Nek9; NimA-related Protein Kinase 9; DKFZp434D0935; HGNC:18591; KIAA1995; MGC138306; MGC16714; Nercc1 Kinase; NERCC1; NERCC; NIMA-related K; Anti -NEK9; anti-NEK9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NEK9. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-NEK9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic peptide selected from the C-terminal region of human NEK9 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-NEK9 Pab is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).)
Western Blot (WB)
(The anti-NEK9 Pab is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-NEK9 antibody
NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
Product Categories/Family for anti-NEK9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,337 Da
NCBI Official Full Name
NEK9 protein
NCBI Official Synonym Full Names
NIMA (never in mitosis gene a)- related kinase 9
NCBI Official Symbol
NEK9
NCBI Official Synonym Symbols
Nek8; NERCC; NERCC1; MGC16714; MGC138306; DKFZp434D0935
NCBI Protein Information
serine/threonine-protein kinase Nek9; nercc1 kinase; nimA-related kinase 8; NIMA-related kinase Nek8; nimA-related protein kinase 9; never in mitosis A-related kinase 9
UniProt Protein Name
NEK9 protein
Protein Family
UniProt Gene Name
NEK9
UniProt Entry Name
Q6PKF2_HUMAN
Uniprot Description
NEK9: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.
Protein type: Autophagy; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NEK family
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein kinase binding; ATP binding
Biological Process: mitosis; cell division; mitotic nuclear envelope disassembly; mitotic cell cycle; protein amino acid phosphorylation
Research Articles on NEK9
Similar Products
Product Notes
The NEK9 nek9 (Catalog #AAA628399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK9, CT (NIMA (Never in Mitosis Gene A)-Related Kinase 9, Never in Mitosis A-related Kinase 9, NIMA-related Kinase Nek9, NimA-related Protein Kinase 9, DKFZp434D0935, HGNC:18591, KIAA1995, MGC138306, MGC16714, Nercc1 Kinase, NERCC1, NERCC, NIMA-related K reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEK9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the NEK9 nek9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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