Western Blot (WB)
(Western blot analysis of anti-NEK11L Pab in mouse brain tissue lysate. NEK11L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse NEK11L Polyclonal Antibody
NEK11L, CT (NIMA-related Kinase 11L, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, NEK11, FLJ23495, Serine/threonine Protein Kinase Nek11)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of anti-NEK11L Pab in mouse brain tissue lysate. NEK11L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-NEK11L Pab in mouse brain tissue lysate. NEK11L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(NEK11L Antibody (E495) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NEK11L antibody detected the NEK11L protein (arrow).)
Western Blot (WB)
(NEK11L Antibody (E495) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NEK11L antibody detected the NEK11L protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
NEK11: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. Interacts with isoform 1 of NEK2. Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc. Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Nucleolus; EC 2.7.11.1; Other group; NEK family
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: nucleoplasm; nucleolus
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding
Biological Process: intra-S DNA damage checkpoint; G2/M transition DNA damage checkpoint; protein amino acid phosphorylation
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Product Notes
The NEK11L (Catalog #AAA623744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK11L, CT (NIMA-related Kinase 11L, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, NEK11, FLJ23495, Serine/threonine Protein Kinase Nek11) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEK11L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the NEK11L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK11L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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