Rabbit NEIL1 Polyclonal Antibody | anti-NEIL1 antibody

NEIL1 polyclonal antibody

Cat. Num. AAA3014818

• Gene Names: NEIL1; FPG1; NEI1; hFPG1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: NEIL1; Polyclonal Antibody; NEIL1 polyclonal antibody; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1; anti-NEIL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NEIL1 polyclonal antibody detects endogenous levels of NEIL1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NEIL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human NEIL1
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of NEIL1 polyclonal antibody at 1:500 dilutionLaneA: PC12 whole cell lysateLaneB: AML-12 whole cell lysateLaneC: MCF-7 whole cell lysateLaneD: A549 whole cell lysate)

Related Product Information for anti-NEIL1 antibody

NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1, 2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG familiy of proteins. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2. NEIL1 is a ubiquitiously expressed protein that is upregulated during S phase. NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis.

NCBI and Uniprot Product Information

• NCBI GI #: 375065857
• NCBI GeneID: 79661
• NCBI Accession #: NP_001243481.1
• NCBI GenBank Nucleotide #: NM_001256552.1
• UniProt Accession #: Q96FI4; Q6ZRA7; Q86XW7; Q9H6C3; D3DW75
• Molecular Weight: 43,684 Da
• NCBI Official Full Name: endonuclease 8-like 1 isoform 1
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: NEIL1
• NCBI Official Synonym Symbols: FPG1; NEI1; hFPG1
• NCBI Protein Information: endonuclease 8-like 1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL1
• UniProt Synonym Gene Names: NEH1
• UniProt Entry Name: NEIL1_HUMAN

NCBI Description

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: DNA repair, damage; Lyase; EC 4.2.99.18; Hydrolase

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds

Biological Process: base-excision repair; nucleotide-excision repair; response to oxidative stress; DNA catabolic process, endonucleolytic; negative regulation of nuclease activity