Rabbit ADCY9 Polyclonal Antibody | anti-ADCY9 antibody

ADCY9 Polyclonal Antibody

Cat. Num. AAA3005644

• Gene Names: ADCY9; AC9; ACIX
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADCY9; Polyclonal Antibody; ADCY9 Polyclonal Antibody; Adenylate cyclase type 9; ATP pyrophosphate-lyase 9; Adenylate cyclase type IX; Adenylyl cyclase 9; KIAA0520; anti-ADCY9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADCY9 polyclonal antibody detects endogenous levels of ADCY9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1353

• Applicable Applications for anti-ADCY9 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ADCY9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADCY9 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY9 pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY9 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY9 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ADCY9 antibody

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules (primarily cyclic AMP-dependent protein kinases) to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Classically, adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor (i.e., alpha beta-adrenergic receptor) catalyzes the exchange of GDP (bound to Galpha s) for GTP, dissociation of GTP-Galpha s from Gbeta gamma and Galpha s-mediated activation of adenylyl cyclase. The most abundant cerebral adenylyl cyclases appears to be adenylyl cyclase IX. AC IX is confined to the gray matter and its expression is mainly neuronal, with its highest expression located at the hippocampus. ACIX is also expressed in heart, pancreas and thyrocytes. AC I and AC IX are regulated reciprocally by intracellular free Ca2+. The inhibition of AC IX by Ca2+ is blocked by the calcineurin inhibitors FK506 and cyclosporin A.

NCBI and Uniprot Product Information

• NCBI GI #: 50959205
• NCBI GeneID: 115
• NCBI Accession #: NP_001107.2
• NCBI GenBank Nucleotide #: NP_001107.2
• UniProt Accession #: O60503
• Molecular Weight: ~ 161kDa
• NCBI Official Full Name: adenylate cyclase type 9
• NCBI Official Synonym Full Names: adenylate cyclase 9
• NCBI Official Symbol: ADCY9
• NCBI Official Synonym Symbols: AC9; ACIX
• NCBI Protein Information: adenylate cyclase type 9
• UniProt Protein Name: Adenylate cyclase type 9
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY9
• UniProt Synonym Gene Names: KIAA0520
• UniProt Entry Name: ADCY9_HUMAN

NCBI Description

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY9: May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Transporter; Adenylyl cyclase; Lyase; Membrane protein, integral; Membrane protein, multi-pass; Transporter, aquaporin family; EC 4.6.1.1; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: metal ion binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; synaptic transmission; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; transmembrane transport; G-protein signaling, adenylate cyclase activating pathway