Goat anti-Human NEIL1 Polyclonal Antibody | anti-NEIL1 antibody

NEIL1, CT (Endonuclease 8-like 1, DNA Glycosylase/AP Lyase Neil1, DNA-(Apurinic or Apyrimidinic Site) Lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei Homolog 1, NEH1, Nei-like Protein 1)

Cat. Num. AAA6005571

• Gene Names: NEIL1; FPG1; NEI1; hFPG1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NEIL1; Polyclonal Antibody; CT (Endonuclease 8-like 1; DNA Glycosylase/AP Lyase Neil1; DNA-(Apurinic or Apyrimidinic Site) Lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei Homolog 1; NEH1; Nei-like Protein 1); Anti -NEIL1; anti-NEIL1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human NEIL1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-NEIL1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-DIPSLEPEGTSAS from the C-terminal of human NEIL1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta (formalin-fixed, paraffin-embedded) stained with NEIL1 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-NEIL1 antibody

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).

Product Categories/Family for anti-NEIL1 antibody

Antibodies; Abs to Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 14790078
• NCBI GeneID: 79661
• UniProt Accession #: Q96FI4; Q6ZRA7; Q86XW7; Q9H6C3; D3DW75
• Molecular Weight: 43,684 Da
• NCBI Official Full Name: NEIL1 protein
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: NEIL1
• NCBI Official Synonym Symbols: FPG1; NEI1; hFPG1
• NCBI Protein Information: endonuclease 8-like 1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL1
• UniProt Synonym Gene Names: NEH1
• UniProt Entry Name: NEIL1_HUMAN

NCBI Description

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: Lyase; Hydrolase; EC 4.2.99.18; DNA repair, damage

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds

Biological Process: nucleotide-excision repair; base-excision repair; response to oxidative stress; DNA catabolic process, endonucleolytic; negative regulation of nuclease activity