Western Blot (WB)
(Western Blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.)
Rabbit NEFM Polyclonal Antibody | anti-NEFM antibody
NEFM Polyclonal Antibody
ICC: 1:100-1:500
IHC: 1:50-1:200
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: axon; neurofilament; neuromuscular junction
Molecular Function: protein binding; structural constituent of cytoskeleton; microtubule binding
Biological Process: neurofilament bundle assembly; regulation of axon diameter; axon cargo transport; microtubule cytoskeleton organization and biogenesis
Research Articles on NEFM
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Product Notes
The NEFM nefm (Catalog #AAA3013111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEFM Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEFM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1,000-1:2,000 ICC: 1:100-1:500 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NEFM nefm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEFM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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