Rabbit anti-Mouse, Rat NEFA/NUCB2 Polyclonal Antibody | anti-NEFA/NUCB2 antibody

Rabbit anti NEFA/NUCB2 Polyclonal Antibody

Cat. Num. AAA460342

• Gene Names: NUCB2; NEFA; HEL-S-109
• Reactivity: Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: NEFA/NUCB2; Polyclonal Antibody; Rabbit anti NEFA/NUCB2 Polyclonal Antibody; Nucleobindin 2; anti-NEFA/NUCB2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~54 kDa of NEFAS protein. It reacts with rat and mouse. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 420

• Applicable Applications for anti-NEFA/NUCB2 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the internal sequence (a portion from 120aa-180aa) of mouse NEFA protein. This sequence is identical to mouse and rat.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-NEFA/NUCB2 antibody

NEFA, a 420 amino acid protein (also known as NUCB2, nucleobindin 2, p54), is a DNA-binding protein localizing in the Golgi Apparatus. This protein has been implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding and TNF-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and ARTS-1, both through the EF-hand motif. The NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been identified as a novel satiety molecule in the hypothalamous associated with melanocortin signaling in the central nervous system.NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.

References

Taniguchi, N et al, The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 275 (41), 31674-31681 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 1208397293
• NCBI GeneID: 4925
• NCBI Accession #: NP_001339601.1
• NCBI GenBank Nucleotide #: NM_001352672.1
• Molecular Weight: 50,201 Da
• NCBI Official Full Name: nucleobindin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: nucleobindin 2
• NCBI Official Symbol: NUCB2
• NCBI Official Synonym Symbols: NEFA; HEL-S-109
• NCBI Protein Information: nucleobindin-2
• UniProt Protein Name: Nucleobindin-2
• UniProt Gene Name: NUCB2
• UniProt Synonym Gene Names: NEFA

NCBI Description

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Uniprot Description

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol; ER-Golgi intermediate compartment; extracellular space; plasma membrane

Molecular Function: protein binding