Rabbit Nedd4 Polyclonal Antibody | anti-Nedd4 antibody

Nedd4, WW2 domain (MaxLight 650)

Cat. Num. AAA6489547

• Gene Names: NEDD4; RPF1; NEDD4-1
• Reactivity: Rat, Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nedd4; Polyclonal Antibody; WW2 domain (MaxLight 650); anti-Nedd4 antibody

• Host: Rabbit
• Reactivity: Rat, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Nedd4, Mr ~115kD. Degradation products were also detected at Mr 55 and 60kD. Species Crossreactivity: mouse and human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-Nedd4 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections.; Applications are based on unconjugated antibody.; WB: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol. Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with.
• Immunogen: GST fusion protein corresponding to aa395-462 of the WW2 domain of rat Nedd4 (NM_006154).
• Conjugate: MaxLight650
• Positive Control: Kidney cytoplasmic extracts
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Mouse kidney cytoplasmic extracts probed with MBS614066 (1:10,000).)

Product Categories/Family for anti-Nedd4 antibody

Antibodies; Proteins

References

1. Anan, T., et al., Genes to Cells 3: 751-763 (1998). 2. Staub, O., et al., American Journal of Physiology 272: C1871-C1880 (1997). 3. Cristillo, A.D., et al., J. Biol. Chem. 278: 34, 587-34, 597 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 313104311
• NCBI GeneID: 4734
• NCBI Accession #: P46934.4
• NCBI GenBank Nucleotide #: NM_006154
• UniProt Accession #: P46934; A1KY35; A6ND72; A7MD29; B4E2R7; B7ZM59; B7ZM60; B9EGN5; D6RF89
• Molecular Weight: 1319
• NCBI Official Full Name: E3 ubiquitin-protein ligase NEDD4
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• NCBI Official Symbol: NEDD4
• NCBI Official Synonym Symbols: RPF1; NEDD4-1
• NCBI Protein Information: E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: NEDD4
• UniProt Synonym Gene Names: KIAA0093; NEDD4-1; NEDD-4
• UniProt Entry Name: NEDD4_HUMAN

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).