Rabbit anti-Human NDUFS5 Polyclonal Antibody | anti-NDUFS5 antibody

NDUFS5 (E89) Polyclonal Antibody

Cat. Num. AAA3013726

• Gene Names: NDUFS5; CI15K; CI-15k
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NDUFS5; Polyclonal Antibody; NDUFS5 (E89) Polyclonal Antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; Complex I-15 kDa; CI-15 kDa; NADH-ubiquinone oxidoreductase 15 kDa subunit; anti-NDUFS5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NDUFS5 (E89) polyclonal antibody detects endogenous levels of NDUFS5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 106

• Applicable Applications for anti-NDUFS5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 61-110 of Human NDUFS5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NDUFS5 (E89) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of NDUFS5 (E89) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-NDUFS5 antibody

Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. By oxidizing NADH that is produced in the Krebs cycle, this complex utilizes the two electrons to reduce ubiquinone to ubiquinol, thereby initiating the passage of electrons to successive complexes and ultimately leading to the reduction of oxygen to water. Mitochondrial complex I consists of over 40 subunits and is of considerable clinical interest since defects in any of the subunits can lead to various myopathies and neuropathies. As a subunit of mitochondrial complex I, NDUFS5 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5), also designated NADH-ubiquinone oxidoreductase 15 kDa subunit, is a 106 amino acid protein that is suggested to not be involved in catalysis. NDUFS5 is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart.

NCBI and Uniprot Product Information

• NCBI GI #: 297206823
• NCBI GeneID: 4725
• NCBI Accession #: NP_001171908.1
• NCBI GenBank Nucleotide #: NP_001171908.1
• UniProt Accession #: O43920
• Molecular Weight: ~ 15kDa
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit S5
• NCBI Official Symbol: NDUFS5
• NCBI Official Synonym Symbols: CI15K; CI-15k
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
• Protein Family: NADH dehydrogenase [ubiquinone] iron-sulfur protein
• UniProt Gene Name: NDUFS5
• UniProt Synonym Gene Names: CI-15 kDa
• UniProt Entry Name: NDUS5_HUMAN

NCBI Description

This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq, May 2010]

Uniprot Description

NDUFS5: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFS5 subunit family.

Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial

Chromosomal Location of Human Ortholog: 1p34.2-p33

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial respiratory chain complex I assembly; mitochondrial electron transport, NADH to ubiquinone