Rabbit anti-Human, Mouse NDUFS2 Polyclonal Antibody | anti-NDUFS2 antibody

Rabbit Polyclonal (IgG) to Human NDUFS2

Cat. Num. AAA248231

• Gene Names: NDUFS2; CI-49
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: NDUFS2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human NDUFS2; Anti-NDUFS2 Antibody (aa200-430) IHC-plus; Complex I 49kDa subunit; Complex I-49kD; CI-49; CI-49kD; Human NDUFS2; anti-NDUFS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human NDUFS2
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
• Concentration: 0.89 mg/ml (varies by lot)
• Sequence Length: 463

• Applicable Applications for anti-NDUFS2 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1:100 - 1:1000), IHC-P (7.5 ug/ml), WB (1:1000 - 1:10000); Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Target Species: Human
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 200 and 430 of NDUFS2 (SwissProt O75306).
• Immunogen Type: Recombinant protein
• Immunogen: NDUFS2 antibody was raised against recombinant fragment corresponding to a region within amino acids 200 and 430 of NDUFS2 (SwissProt O75306).
• Antigen Modification: aa200-430
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NDUFS2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. NDUFS2 protein stained by NDUFS2 antibody diluted at 1:500.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A:293T, B: A431, C: JurKat, D: Raji. 10% SDS PAGE. NDUFS2 antibody diluted at 1:1000.)

Western Blot (WB)

(Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. NDUFS2 antibody diluted at 1:10000.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758786
• NCBI GeneID: 4720
• NCBI Accession #: NP_004541.1
• NCBI GenBank Nucleotide #: NM_004550.4
• UniProt Accession #: O75306; Q5VTW0; Q969P3; Q9UEV3; D3DVG7; J3KPM7
• Molecular Weight: 51,852 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS2
• NCBI Official Synonym Symbols: CI-49
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; CI-49kD; complex I-49kD; complex I 49kDa subunit; NADH-ubiquinone oxidoreductase 49 kDa subunit; NADH-ubiquinone oxidoreductase NDUFS2 subunit; complex 1, mitochondrial respiratory chain, 49-KD subunit
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
• UniProt Gene Name: NDUFS2
• UniProt Synonym Gene Names: CI-49kD
• UniProt Entry Name: NDUS2_HUMAN

NCBI Description

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

NDUFS2: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I 49 kDa subunit family.

Protein type: EC 1.6.99.3; Oxidoreductase; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: protein binding; electron carrier activity; NADH dehydrogenase (ubiquinone) activity; metal ion binding; 4 iron, 4 sulfur cluster binding; NAD binding; quinone binding; NADH dehydrogenase activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; response to oxidative stress

Disease: Mitochondrial Complex I Deficiency