Rabbit anti-Human NDUFC2 Polyclonal Antibody | anti-NDUFC2 antibody

NDUFC2, CT (NDUFC2, NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B14.5b, Human lung cancer oncogene 1 protein, NADH-ubiquinone oxidoreductase subunit B14.5b) (PE)

Cat. Num. AAA6324429

• Gene Names: NDUFC2; HLC-1; B14.5b; NADHDH2; CI-B14.5b
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFC2; Polyclonal Antibody; CT (NDUFC2; NADH dehydrogenase [ubiquinone] 1 subunit C2; Complex I-B14.5b; Human lung cancer oncogene 1 protein; NADH-ubiquinone oxidoreductase subunit B14.5b) (PE); NADH-ubiquinone oxidoreductase subunit B14.5b; anti-NDUFC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 119

• Applicable Applications for anti-NDUFC2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NDUFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the C-terminal region of human NDUFC2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(NDUFC2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(NDUFC2 Antibody (C-term) western blot analysis in MDA-MB453,Hela,293 cell line lysates (35ug/lane).This demonstrates the NDUFC2 antibody detected the NDUFC2 protein (arrow).)

Related Product Information for anti-NDUFC2 antibody

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Categories/Family for anti-NDUFC2 antibody

Antibodies; Cancer Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758784
• NCBI GeneID: 4718
• NCBI Accession #: NP_004540.1
• NCBI GenBank Nucleotide #: NP_004540.1
• UniProt Accession #: O95298
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit C2
• NCBI Official Symbol: NDUFC2
• NCBI Official Synonym Symbols: HLC-1; B14.5b; NADHDH2; CI-B14.5b
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 subunit C2
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 subunit C2
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFC2
• UniProt Synonym Gene Names: CI-B14.5b; HLC-1
• UniProt Entry Name: NDUC2_HUMAN

Uniprot Description

NDUFC2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFC2 subunit family.

Protein type: Membrane protein, integral; Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: mitochondrial inner membrane; cytoplasm; integral to membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone