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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NDUFB5 antibody.)

Rabbit NDUFB5 Polyclonal Antibody | anti-NDUFB5 antibody

NDUFB5 Polyclonal Antibody

Gene Names
NDUFB5; SGDH; CISGDH
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antigen Affinity Purification
Synonyms
NDUFB5; Polyclonal Antibody; NDUFB5 Polyclonal Antibody; SGDH; CISGDH; anti-NDUFB5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Sequence Length
53
Applicable Applications for anti-NDUFB5 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human NDUFB5
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-100060 / sc-100059 / sc-133807
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NDUFB5 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NDUFB5 antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
22kDa
NCBI Official Full Name
NDUFB5 protein
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B5
NCBI Official Symbol
NDUFB5
NCBI Official Synonym Symbols
SGDH; CISGDH
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
Protein Family
UniProt Gene Name
NDUFB5
UniProt Synonym Gene Names
CI-SGDH
UniProt Entry Name
NDUB5_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

NDUFB5: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB5 subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.5.3; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleoplasm

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly

Research Articles on NDUFB5

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Product Notes

The NDUFB5 ndufb5 (Catalog #AAA2540715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB5 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the NDUFB5 ndufb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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