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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat and A549 cell, human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Rabbit anti-Human NDUFB10 Polyclonal Antibody | anti-NDUFB10 antibody

NDUFB10 Antibody

Gene Names
NDUFB10; PDSW
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
NDUFB10; Polyclonal Antibody; NDUFB10 Antibody; PDSW; anti-NDUFB10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NDUFB10 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
2 mg/ml (varies by lot)
Sequence Length
172
Applicable Applications for anti-NDUFB10 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:25-1:100
Immunogen
Full length fusion protein of human NDUFB10
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat and A549 cell, human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat and A549 cell, human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/35.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/35.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/35.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/35.)
Related Product Information for anti-NDUFB10 antibody
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 is an enzyme that in humans is encoded by theNDUFB10 gene.NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 10 is an accessory subunit of the NADH dehydrogenase (ubiquinone) complex, located in the mitochondrial inner membrane. It is also known as Complex I and is the largest of the five complexes of the electron transport chain.
Product Categories/Family for anti-NDUFB10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,143 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B10
NCBI Official Symbol
NDUFB10
NCBI Official Synonym Symbols
PDSW
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
UniProt Gene Name
NDUFB10
UniProt Synonym Gene Names
CI-PDSW
UniProt Entry Name
NDUBA_HUMAN

Uniprot Description

NDUFB10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB10 subunit family.

Protein type: EC 1.6.5.3; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: protein binding

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly

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Product Notes

The NDUFB10 ndufb10 (Catalog #AAA9415338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the NDUFB10 ndufb10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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