Western Blot (WB)
(Western Blot (WB) analysis of NDUFB1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Hela whole cell lysate)
Rabbit NDUFB1 Polyclonal Antibody | anti-NDUFB1 antibody
NDUFB1 Polyclonal Antibody
Gene Names
NDUFB1; MNLL; CI-MNLL; CI-SGDH
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NDUFB1; Polyclonal Antibody; NDUFB1 Polyclonal Antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; Complex I-MNLL; CI-MNLL; NADH-ubiquinone oxidoreductase MNLL subunit; anti-NDUFB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NDUFB1 polyclonal antibody detects endogenous levels of NDUFB1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
105
Applicable Applications for anti-NDUFB1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human NDUFB1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NDUFB1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Hela whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of NDUFB1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Hela whole cell lysate)
Related Product Information for anti-NDUFB1 antibody
NDUFB1 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1), also known as CI-MNLL (complex I-MNLL), CI-SGDH or NADH-ubiquinone oxidoreductase MNLL subunit, is a 58 amino acid single-pass membrane protein that localizes to the matrix side of the mitochondrial membrane. A member of the complex I NDUFB1 subunit family, NDUFB1 is encoded by a gene that maps to human chromosome 14q32.12. Chromosome 14 houses over 700 genes and comprises nearly 3.5% of the human genome. Chromosome 14 encodes the presinilin 1 (PSEN1) gene, which is one of the three key genes associated with the development of Alzheimer's disease (AD). The SERPINA1 gene is also located on chromosome 14 and, when defective, leads to the genetic disorder alpha1-antitrypsin deficiency, which is characterized by severe lung complications and liver dysfunction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 12kDa
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B1
NCBI Official Symbol
NDUFB1
NCBI Official Synonym Symbols
MNLL; CI-MNLL; CI-SGDH
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
Protein Family
UniProt Gene Name
NDUFB1
UniProt Synonym Gene Names
CI-MNLL
UniProt Entry Name
NDUB1_HUMAN
Uniprot Description
NDUFB1: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB1 subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q32.12
Cellular Component: mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain complex I
Molecular Function: NADH dehydrogenase (ubiquinone) activity
Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
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Product Notes
The NDUFB1 ndufb1 (Catalog #AAA3007269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the NDUFB1 ndufb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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