Rabbit anti-Human NDUFAF4 Polyclonal Antibody | anti-NDUFAF4 antibody

NDUFAF4, NT (NDUFAF4, C6orf66, HRPAP20, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, Hormone-regulated proliferation-associated protein of 20kD) (PE)

Cat. Num. AAA6324418

• Gene Names: NDUFAF4; My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFAF4; Polyclonal Antibody; NT (NDUFAF4; C6orf66; HRPAP20; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4; Hormone-regulated proliferation-associated protein of 20kD) (PE); Hormone-regulated proliferation-associated protein of 20kD; anti-NDUFAF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NDUFAF4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NDUFAF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human NDUFAF4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NDUFAF4 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NDUFAF4 antibody detected the NDUFAF4 protein (arrow).)

Immunohistochemistry (IHC)

(NDUFAF4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFAF4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NDUFAF4 antibody

Antibodies; KIAA; orf

References

Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009). Zimmermann, F.A., et al. Br. J. Cancer 100(9):1434-1437(2009). Saada, A., et al. Am. J. Hum. Genet. 82(1):32-38(2008). Tozlu-Kara, S., et al. J. Mol. Endocrinol. 39(4):305-318(2007). Karp, C.M., et al. Oncogene 26(12):1780-1788(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 7661786
• NCBI GeneID: 29078
• NCBI Accession #: NP_054884.1
• NCBI GenBank Nucleotide #: NM_014165.3
• UniProt Accession #: Q9P032; B2R4J5
• Molecular Weight: 20,266 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) complex I, assembly factor 4
• NCBI Official Symbol: NDUFAF4
• NCBI Official Synonym Symbols: My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4; hormone-regulated proliferation-associated p
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFAF4
• UniProt Synonym Gene Names: C6orf66; HRPAP20
• UniProt Entry Name: NDUF4_HUMAN

NCBI Description

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. [provided by RefSeq, Oct 2009]

Uniprot Description

NDUFAF4: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. Defects in NDUFAF4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the NDUFAF4 family.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: mitochondrion; mitochondrial membrane

Molecular Function: calmodulin binding; protein binding

Biological Process: mitochondrial respiratory chain complex I assembly

Disease: Mitochondrial Complex I Deficiency