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Immunohistochemistry (IHC) (IHC image of MBS7109548 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Rabbit anti-Human NDUFAF3 Polyclonal Antibody | anti-NDUFAF3 antibody

NDUFAF3 Antibody

Gene Names
NDUFAF3; 2P1; E3-3; C3orf60
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
NDUFAF3; Polyclonal Antibody; NDUFAF3 Antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3; C3orf60; anti-NDUFAF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
184
Applicable Applications for anti-NDUFAF3 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC: 1:20-1:200
Immunogen
Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 protein (91-184AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image of MBS7109548 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7109548 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7109548 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7109548 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-NDUFAF3 antibody
Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,842 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase complex assembly factor 3
NCBI Official Symbol
NDUFAF3
NCBI Official Synonym Symbols
2P1; E3-3; C3orf60
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
Protein Family
UniProt Gene Name
NDUFAF3
UniProt Synonym Gene Names
C3orf60

NCBI Description

This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2009]

Uniprot Description

Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).

Research Articles on NDUFAF3

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Product Notes

The NDUFAF3 ndufaf3 (Catalog #AAA7109548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFAF3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFAF3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). IHC: 1:20-1:200. Researchers should empirically determine the suitability of the NDUFAF3 ndufaf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFAF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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