Rabbit anti-Human, Mouse NDUF3 Polyclonal Antibody | anti-NDUF3 antibody

NDUF3, NT (NDUFAF3, C3orf60, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3) (FITC)

Cat. Num. AAA6324345

• Gene Names: NDUFAF3; 2P1; E3-3; C3orf60; MC1DN18
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUF3; Polyclonal Antibody; NT (NDUFAF3; C3orf60; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3) (FITC); NDUFAF3; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3; anti-NDUF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NDUF3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NDUF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human NDUF3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUF3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NDUF3 antibody detected the NDUF3 protein (arrow).)

Immunohistochemistry (IHC)

(NDUF3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUF3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NDUF3 antibody

This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-NDUF3 antibody

Antibodies; KIAA; orf

References

Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009). Hammami-Hamza, S., et al. Int. J. Dev. Biol. 47(1):71-76(2003). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 41327781
• NCBI GeneID: 25915
• NCBI Accession #: NP_951032.1
• NCBI GenBank Nucleotide #: NM_199069.1
• UniProt Accession #: Q9BU61
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase complex assembly factor 3
• NCBI Official Symbol: NDUFAF3
• NCBI Official Synonym Symbols: 2P1; E3-3; C3orf60; MC1DN18
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
• UniProt Gene Name: NDUFAF3
• UniProt Synonym Gene Names: C3orf60

NCBI Description

This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2009]

Uniprot Description

Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).