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SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: MBS7126021(NDUFAF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Rabbit anti-Human, Mouse NDUFAF1 Polyclonal Antibody | anti-NDUFAF1 antibody

NDUFAF1 Antibody

Gene Names
NDUFAF1; CGI65; CIA30; CGI-65
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Antigen Affinity Purification
Synonyms
NDUFAF1; Polyclonal Antibody; NDUFAF1 Antibody; NADH dehydrogenase (ubiquinone) complex I; assembly factor 1; anti-NDUFAF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Applicable Applications for anti-NDUFAF1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:2000-1:5000
WB: 1:500-1:2000
Immunogen
Fusion protein of human NDUFAF1
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

SDS-PAGE

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: MBS7126021(NDUFAF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: MBS7126021(NDUFAF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Product Categories/Family for anti-NDUFAF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,764 Da
NCBI Official Full Name
complex I intermediate-associated protein 30, mitochondrial
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) complex I, assembly factor 1
NCBI Official Symbol
NDUFAF1
NCBI Official Synonym Symbols
CGI65; CIA30; CGI-65
NCBI Protein Information
complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, assembly factor 1
UniProt Protein Name
Complex I intermediate-associated protein 30, mitochondrial
UniProt Gene Name
NDUFAF1
UniProt Synonym Gene Names
CIA30
UniProt Entry Name
CIA30_HUMAN

NCBI Description

This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]

Uniprot Description

NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family.

Chromosomal Location of Human Ortholog: 15q11.2-q21.3

Cellular Component: cytoplasm; mitochondrial respiratory chain complex I

Molecular Function: protein binding; unfolded protein binding

Biological Process: mitochondrial electron transport, NADH to ubiquinone; protein complex assembly

Disease: Mitochondrial Complex I Deficiency

Research Articles on NDUFAF1

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Product Notes

The NDUFAF1 ndufaf1 (Catalog #AAA7126021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFAF1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFAF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:2000-1:5000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NDUFAF1 ndufaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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