Western Blot (WB)
(Western Blot analysis of NDST4 expression in transfected 293T cell line by NDST4 polyclonal antibody. Lane 1: NDST4 transfected lysate (100.7kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human NDST4 Polyclonal Antibody | anti-NDST4 antibody
NDST4 (HSST4, Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 4, Glucosaminyl N-deacetylase/N-sulfotransferase 4, N-heparan Sulfate Sulfotransferase 4, Heparan Sulfate N-deacetylase 4, Heparan Sulfate N-sulfotransferase 4) (FITC)
Gene Names
NDST4; NDST-4; NHSST4
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NDST4; Polyclonal Antibody; NDST4 (HSST4; Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 4; Glucosaminyl N-deacetylase/N-sulfotransferase 4; N-heparan Sulfate Sulfotransferase 4; Heparan Sulfate N-deacetylase 4; Heparan Sulfate N-sulfotransferase 4) (FITC); anti-NDST4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NDST4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-NDST4 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human NDST4, aa1-872 (NP_072091.1).
Immunogen Sequence
MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of NDST4 expression in transfected 293T cell line by NDST4 polyclonal antibody. Lane 1: NDST4 transfected lysate (100.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NDST4 expression in transfected 293T cell line by NDST4 polyclonal antibody. Lane 1: NDST4 transfected lysate (100.7kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-NDST4 antibody
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity).
Product Categories/Family for anti-NDST4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
100,716 Da
NCBI Official Full Name
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
NCBI Official Synonym Full Names
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4
NCBI Official Symbol
NDST4
NCBI Official Synonym Symbols
NDST-4; NHSST4
NCBI Protein Information
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4; N-HSST 4; N-heparan sulfate sulfotransferase 4; glucosaminyl N-deacetylase/N-sulfotransferase 4
UniProt Protein Name
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
UniProt Gene Name
NDST4
UniProt Synonym Gene Names
HSST4; NDST-4; N-HSST 4
UniProt Entry Name
NDST4_HUMAN
Uniprot Description
NDST4: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity. Belongs to the sulfotransferase 1 family. NDST subfamily.
Protein type: EC 2.8.2.8; Glycan Metabolism - heparan sulfate biosynthesis; Hydrolase; Transferase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q26
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: deacetylase activity; [heparan sulfate]-glucosamine N-sulfotransferase activity
Biological Process: heparin biosynthetic process; heparan sulfate proteoglycan biosynthetic process
Similar Products
Product Notes
The NDST4 ndst4 (Catalog #AAA6386603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDST4 (HSST4, Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 4, Glucosaminyl N-deacetylase/N-sulfotransferase 4, N-heparan Sulfate Sulfotransferase 4, Heparan Sulfate N-deacetylase 4, Heparan Sulfate N-sulfotransferase 4) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDST4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NDST4 ndst4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDST4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
