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Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: NDRG4 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39, 38, 41, 43, 40 KDaObserved band size: 39 KDa)

Rabbit anti-Human NDRG4 Polyclonal Antibody | anti-NDRG4 antibody

NDRG4 Antibody

Gene Names
NDRG4; BDM1; SMAP8; SMAP-8
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
NDRG4; Polyclonal Antibody; NDRG4 Antibody; Protein NDRG4; Brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; Vascular smooth muscle cell-associated protein 8; SMAP-8; BDM1; KIAA1180; anti-NDRG4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
391
Applicable Applications for anti-NDRG4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Protein NDRG4 protein (210-331AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: NDRG4 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39, 38, 41, 43, 40 KDaObserved band size: 39 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: NDRG4 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39, 38, 41, 43, 40 KDaObserved band size: 39 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7105457 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human heart tissue using MBS7105457 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS7105457 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7105457 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7105457 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7105457 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-NDRG4 antibody
Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress (By similarity). May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.
Product Categories/Family for anti-NDRG4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,168 Da
NCBI Official Full Name
protein NDRG4 isoform 2
NCBI Official Synonym Full Names
NDRG family member 4
NCBI Official Symbol
NDRG4
NCBI Official Synonym Symbols
BDM1; SMAP8; SMAP-8
NCBI Protein Information
protein NDRG4
UniProt Protein Name
Protein NDRG4
Protein Family
UniProt Gene Name
NDRG4
UniProt Synonym Gene Names
BDM1; KIAA1180; SMAP-8

NCBI Description

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]

Uniprot Description

Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress (). May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.

Research Articles on NDRG4

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Product Notes

The NDRG4 ndrg4 (Catalog #AAA7105457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDRG4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the NDRG4 ndrg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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