anti-Rat NCX1 Polyclonal Antibody | anti-NCX1 antibody
Anti - NCX1 (Sodium Calcium Exchanger)
Gene Names
SLC8A1; NCX1; FLJ37694; FLJ43417; MGC119581; DKFZp779F0871
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
NCX1; Polyclonal Antibody; Anti - NCX1 (Sodium Calcium Exchanger); NCX1 (Sodium Calcium Exchanger); anti-NCX1 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The 23 AA rat control peptide 100% conserved in mouse, feline, 95% in human, rabbit, monkey, bovine, chicken and 73% in frog NCX1. No significant sequence homology is detected with other NCX. The control peptide is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
84
Applicable Applications for anti-NCX1 antibody
ELISA, Western Blot
Application Notes
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 23 AA Peptide sequence within the cytoplasmic domain 3 of rat NCX1 (1). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NCX1 antibody
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.
Product Categories/Family for anti-NCX1 antibody
References
• 1. Low, W., et al. (1993) FEBS Lett. 316, 63.
• 2. Furman, I., et al. (1993) FEBS Lett. 319, 105.
• 3. Lee, S.L., et al. (1994) J Biol. Chem. 269, 14849.
• 4. Nicoll, D.A., et al. (1996) J Biol. Chem. 271, 24914.
• 5. Kumoro, I., et al. (1992) PNAS 89, 4769.
• 6. Blaustein, M.P. and Lederer, J. (1999) Physiol Rev. 79, 763-854 (review).
• 2. Furman, I., et al. (1993) FEBS Lett. 319, 105.
• 3. Lee, S.L., et al. (1994) J Biol. Chem. 269, 14849.
• 4. Nicoll, D.A., et al. (1996) J Biol. Chem. 271, 24914.
• 5. Kumoro, I., et al. (1992) PNAS 89, 4769.
• 6. Blaustein, M.P. and Lederer, J. (1999) Physiol Rev. 79, 763-854 (review).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
9,406 Da
NCBI Official Full Name
ncx1
NCBI Official Synonym Full Names
solute carrier family 8 (sodium/calcium exchanger), member 1
NCBI Official Symbol
SLC8A1
NCBI Official Synonym Symbols
NCX1; FLJ37694; FLJ43417; MGC119581; DKFZp779F0871
NCBI Protein Information
sodium/calcium exchanger 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; OTTHUMP00000201546; OTTHUMP00000201569; OTTHUMP00000201570; OTTHUMP00000201587; OTTHUMP00000201589; Na(+)/Ca(2+)-exchange protein 1
UniProt Protein Name
Ncx1 protein
UniProt Gene Name
ncx1
UniProt Entry Name
Q6LAJ8_HUMAN
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Product Notes
The NCX1 ncx1 (Catalog #AAA617231) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - NCX1 (Sodium Calcium Exchanger) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCX1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NCX1 ncx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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