Rabbit anti-Human NCOA4 Polyclonal Antibody | anti-NCOA4 antibody

NCOA4, CT (NCOA4, ARA70, ELE1, RFG, Nuclear receptor coactivator 4, Androgen receptor coactivator 70kD protein, Androgen receptor-associated protein of 70kD, Ret-activating protein ELE1) (FITC)

Cat. Num. AAA6324202

• Gene Names: NCOA4; RFG; ELE1; PTC3; ARA70
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCOA4; Polyclonal Antibody; CT (NCOA4; ARA70; ELE1; RFG; Nuclear receptor coactivator 4; Androgen receptor coactivator 70kD protein; Androgen receptor-associated protein of 70kD; Ret-activating protein ELE1) (FITC); Ret-activating protein ELE1; anti-NCOA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 614

• Applicable Applications for anti-NCOA4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NCOA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-524 amino acids from the C-terminal region of human NCOA4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NCOA4 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NCOA4 antibody detected the NCOA4 protein (arrow).)

Related Product Information for anti-NCOA4 antibody

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma.

Product Categories/Family for anti-NCOA4 antibody

Antibodies; Receptors

References

Sadow, P.M., et al. Endocr. Pathol. 21(2):73-79(2010). Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009): Richardson, D.S., et al. Cancer Res. 69(11):4861-4869(2009). Peng, Y., et al. Am. J. Pathol. 172(1):225-235(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 14149617
• NCBI GeneID: 8031
• NCBI Accession #: NP_005428.1; NP_001138732.1; NP_001138734.1
• NCBI GenBank Nucleotide #: NP_005428.1
• UniProt Accession #: Q13772
• NCBI Official Full Name: nuclear receptor coactivator 4 isoform 3
• NCBI Official Synonym Full Names: nuclear receptor coactivator 4
• NCBI Official Symbol: NCOA4
• NCBI Official Synonym Symbols: RFG; ELE1; PTC3; ARA70
• NCBI Protein Information: nuclear receptor coactivator 4
• UniProt Protein Name: Nuclear receptor coactivator 4
• Protein Family: Nuclear receptor coactivator
• UniProt Gene Name: NCOA4
• UniProt Synonym Gene Names: ARA70; ELE1; RFG; NCoA-4; 70 kDa AR-activator
• UniProt Entry Name: NCOA4_HUMAN

NCBI Description

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]

Uniprot Description

NCOA4: Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Oncoprotein

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: nucleus

Molecular Function: androgen receptor binding; transcription coactivator activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; male gonad development; response to hormone stimulus

Disease: Thyroid Carcinoma, Papillary