Rabbit anti-Human NCK2 Polyclonal Antibody | anti-NCK2 antibody

NCK2, NT (NCK2, GRB4, Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta) (Azide free) (HRP)

Cat. Num. AAA6324137

• Gene Names: NCK2; GRB4; NCKbeta
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCK2; Polyclonal Antibody; NT (NCK2; GRB4; Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta) (Azide free) (HRP); SH2/SH3 adaptor protein NCK-beta; anti-NCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NCK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NCK2 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the NCK2 antibody detected the NCK2 protein (arrow).)

Immunohistochemistry (IHC)

(NCK2 Antibody (N-term) (AP14107a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NCK2 antibody

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-NCK2 antibody

Antibodies; Growth Factors; Cytokines

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Guan, S., et al. J. Invest. Dermatol. 129(8):1909-1920(2009). Liu, J., et al. PLoS ONE 4 (11), E7805 (2009): Akiyama, M., et al. Br J Ophthalmol 92(9):1293-1296(2008). Park, S., et al. J. Biomol. NMR 34(3):203-208(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 52630425
• NCBI GeneID: 8440
• NCBI Accession #: NP_001004720.1; NP_003572.2
• NCBI GenBank Nucleotide #: NM_001004720.2
• UniProt Accession #: O43639; Q9BWN9; Q9UIC3; D3DVK1
• Molecular Weight: 42,915 Da
• NCBI Official Full Name: cytoplasmic protein NCK2 isoform A
• NCBI Official Synonym Full Names: NCK adaptor protein 2
• NCBI Official Symbol: NCK2
• NCBI Official Synonym Symbols: GRB4; NCKbeta
• NCBI Protein Information: cytoplasmic protein NCK2; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta
• UniProt Protein Name: Cytoplasmic protein NCK2
• Protein Family: Cytoplasmic protein
• UniProt Gene Name: NCK2
• UniProt Synonym Gene Names: GRB4; Nck-2
• UniProt Entry Name: NCK2_HUMAN

NCBI Description

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NCK2: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: endoplasmic reticulum; cytoplasm; vesicle membrane; cytosol

Molecular Function: protein binding; receptor signaling complex scaffold activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity

Biological Process: lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; axon guidance; cell migration; T cell activation; positive regulation of signal transduction; regulation of epidermal growth factor receptor activity; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; signal transduction; regulation of translation; negative regulation of cell proliferation; positive regulation of actin filament polymerization; ephrin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway