Rabbit anti-Human NBN Polyclonal Antibody | anti-NBN antibody

NBN, CT (NBN, NBS, NBS1, P95, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1) (PE)

Cat. Num. AAA6323989

• Gene Names: NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NBN; Polyclonal Antibody; CT (NBN; NBS; NBS1; P95; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1) (PE); Nijmegen breakage syndrome protein 1; anti-NBN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NBN.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NBN antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 602-630 in the C-terminal region of human NBN, UniProt Accession #O60934.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NCI-H46 and NCI-H292 cell line lysates (35ug/lane) usingMBS641463. This demonstrates that MBS641463 detected the NBN protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Hela cell using MBS641463 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS641463 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NBN antibody

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Product Categories/Family for anti-NBN antibody

Antibodies; Disease Markers

References

1. Liu, Y., et al. Carcinogenesis 31(10):1762-1769 (2010). 2. Kavitha, C.V., et al. Biochem. Biophys. Res. Commun. 399 (4):575-580(2010). 3. Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010). 4. Jelonek, K., et al. J. Appl. Genet. 51(3):343-352 (2010). 5. ugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 33356172
• NCBI GeneID: 4683
• NCBI Accession #: NP_002476.2
• NCBI GenBank Nucleotide #: NM_002485.4
• UniProt Accession #: O60934; O60672; Q32NF7; Q53FM6; Q63HR6; Q7LDM2; B2R626; B2RNC5
• Molecular Weight: 84,959 Da
• NCBI Official Full Name: nibrin
• NCBI Official Synonym Full Names: nibrin
• NCBI Official Symbol: NBN
• NCBI Official Synonym Symbols: ATV; NBS; P95; NBS1; AT-V1; AT-V2
• NCBI Protein Information: nibrin; cell cycle regulatory protein p95; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex
• UniProt Protein Name: Nibrin
• Protein Family: Nibrin
• UniProt Gene Name: NBN
• UniProt Synonym Gene Names: NBS; NBS1; P95
• UniProt Entry Name: NBN_HUMAN

NCBI Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Uniprot Description

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; positive regulation of kinase activity; isotype switching; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia