Rabbit anti-Human, Mouse NBN Polyclonal Antibody | anti-NBN antibody

NBN Rabbit pAb

Cat. Num. AAA8544108

• Gene Names: NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: NBN; Polyclonal Antibody; NBN Rabbit pAb; ATV; NBS; P95; NBS1; AT-V1; AT-V2; anti-NBN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
• Sequence Length: 754

• Applicable Applications for anti-NBN antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
• Cellular Localization: Chromosome, Nucleus, Telomere
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-NBN antibody

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.

NCBI and Uniprot Product Information

• NCBI GI #: 33356172
• NCBI GeneID: 4683
• NCBI Accession #: NP_002476.2
• NCBI GenBank Nucleotide #: NP_002476.2
• UniProt Accession #: O60934
• Molecular Weight: 110kDa
• NCBI Official Full Name: nibrin isoform 1
• NCBI Official Synonym Full Names: nibrin
• NCBI Official Symbol: NBN
• NCBI Official Synonym Symbols: ATV; NBS; P95; NBS1; AT-V1; AT-V2
• NCBI Protein Information: nibrin
• UniProt Protein Name: Nibrin
• Protein Family: Nibrin
• UniProt Gene Name: NBN
• UniProt Synonym Gene Names: NBS; NBS1; P95
• UniProt Entry Name: NBN_HUMAN

NCBI Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Uniprot Description

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; positive regulation of kinase activity; isotype switching; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia