anti-Rat NBC1 Polyclonal Antibody | anti-NBC1 antibody

Anti - NBC1 (Na, HCO3 Cotransporter 1, pNBC)

Cat. Num. AAA617220

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum
• Synonyms: NBC1; Polyclonal Antibody; Anti - NBC1 (Na; HCO3 Cotransporter 1; pNBC); NBC1 (Na; anti-NBC1 antibody

• Host: Host: Rabbit; Source: Rat
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rat sequence is 100% conserved in mouse and 94% in human and rabbit NBC1/PSLC4A4/kNBC. This region is unique to the kidney isoforms and it is not found in pancreatic isoform, pNBC/PSLC4A5. No significant sequence homology of was found with NBC2 or NBC3. Antibody crossreactivity in various species is not established. We recommend the use of control peptide in antibody blocking experiments to establish antibody specificity. Control peptides, because of their small size (< 2kD), are not suitable for Western
• Purity/Purification: Serum
• Form/Format: Serum; Supplied as a liquid, neat serum, 40% glycerol.

• Applicable Applications for anti-NBC1 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blotting1-10ug/ml for affinity pure using Chemiluminescence technique. ; ELISA(1:10K-1:100K using 50-100ng of control peptide/well.
• Immunogen: A 19-aa peptide sequence within the cytoplasmic, N-terminus of rat NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH (carrier protein)
• Preparation and Storage: -20 degree C

Related Product Information for anti-NBC1 antibody

Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.

Product Categories/Family for anti-NBC1 antibody

Abs to Ion Transport

References

• Romero MF et al (1998) Am. J. Physiol. 274, F425-F432 Burnham CE et al (1998) gene accession # AF027362; Burnham CE et al (1997) J. Biol. Chem. 272, 19111-19114; Abuladze N et al (1998) J. Biol. Chem. 273; 17689-17695; Giffard RG et al (2000) J. Neurochem. In press; Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review)