anti-Mouse, Sheep Natural Resistance Associated Macrophage Protein Polyclonal Antibody | anti-Slc11a1 antibody
Anti - Natural Resistance Associated Macrophage Protein (Nramp1)
Gene Names
Slc11a1; Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157; Slc11a1
Reactivity
Mouse, Sheep
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Natural Resistance Associated Macrophage Protein; Polyclonal Antibody; Anti - Natural Resistance Associated Macrophage Protein (Nramp1); Natural Resistance Associated Macrophage Protein (Nramp1); anti-Slc11a1 antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
The 21 AA mouse control peptide sequence is 59% conserved in red deer, bison, sheep, bovine, 57% in human NRAMP1. No significant sequence homology exist with NRAMP2. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
548
Applicable Applications for anti-Slc11a1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 21 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of mouse NRAMP1 (1). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Slc11a1 antibody
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human NRAMP1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. NRAMP1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. NRAMP1 (human 550 aa, chromosome 2q35; mouse 548 aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N and C-termini are predicted to be cytoplasmic.
Product Categories/Family for anti-Slc11a1 antibody
References
• (1). Celier M et al (1994) J. Exp med. 180, 1741-1752; Kishi F et al (1995) Immunol. Lett. 47, 93-96; Kihsi F et al (1994) BBRC 204, 1074-1080; Blackwell JM et al (1995) Mol. Med. 1, 194-205; Liu J et al (1995) Am. J. Hum. Genet. 56, 845-853; Barton CH et al (1994) J Exp. Med. 179, 1683-1687; Govoni G et al (1995) Genomics 27, 9-19; Vidal SM et al (1993) Cell 469-485; Cannon-Hergaux F et al (2000) Proc. Assoc. Am. Phys. 111, 283-289 (review), Barton CH et al (1999) J. Leukoc. Biol. 66, 752-762 (review).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,741 Da
NCBI Official Full Name
natural resistance associated macrophage protein
NCBI Official Synonym Full Names
solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
NCBI Official Symbol
Slc11a1
NCBI Official Synonym Symbols
Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157; Slc11a1
NCBI Protein Information
natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh
UniProt Protein Name
Natural resistance-associated macrophage protein 1
Protein Family
UniProt Gene Name
Slc11a1
UniProt Synonym Gene Names
Bcg; Ity; Lsh; Nramp1
UniProt Entry Name
NRAM1_MOUSE
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Product Notes
The Slc11a1 slc11a1 (Catalog #AAA617759) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Natural Resistance Associated Macrophage Protein (Nramp1) reacts with Mouse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Natural Resistance Associated Macrophage Protein can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Slc11a1 slc11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Natural Resistance Associated Macrophage Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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