Rabbit Mcl-1 Polyclonal Antibody | anti-Mcl-1 antibody

RABBIT ANTI HUMAN Mcl-1

Cat. Num. AAA221875

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Applications: Western Blot
• Synonyms: Mcl-1; Polyclonal Antibody; RABBIT ANTI HUMAN Mcl-1; anti-Mcl-1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours. ; ; The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. ; ; This item recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-Mcl-1 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 30kDa and 38kDa in Human Raji cell lysates.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 16 amino acid peptide located near the centre of human MCL-1.; Buffer Solution
• Antiserum Preparation: Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Raji, Burkitt's lymphoma derived B-lymphoblastoid cells probed with Rabbit anti Human Mcl-1 (MBS221875) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunocytochemical staining of Raji cells with Rabbit anti Human Mcl-1 (MBS221875))

Related Product Information for anti-Mcl-1 antibody

MBS221875 recognises an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours. The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. MBS221875 recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.

NCBI and Uniprot Product Information

• NCBI GI #: 309747067
• NCBI GeneID: 4170
• NCBI Accession #: NP_001184249.1
• NCBI GenBank Nucleotide #: NM_001197320.1
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• Molecular Weight: 28,662 Da
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
• NCBI Official Synonym Full Names: myeloid cell leukemia 1
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Channel, misc.; Membrane protein, integral; Apoptosis; Inhibitor; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cell fate determination; cellular homeostasis