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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NAT8 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Rabbit NAT8 Polyclonal Antibody | anti-NAT8 antibody

NAT8 Polyclonal Antibody

Gene Names
NAT8; GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
NAT8; Polyclonal Antibody; NAT8 Polyclonal Antibody; anti-NAT8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
227
Applicable Applications for anti-NAT8 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human NAT8
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NAT8 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NAT8 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using NAT8 antibody (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using NAT8 antibody (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using NAT8 antibody (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using NAT8 antibody (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using NAT8 antibody (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using NAT8 antibody (40x lens).)
Related Product Information for anti-NAT8 antibody
This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26kDa
NCBI Official Full Name
N-acetyltransferase 8
NCBI Official Synonym Full Names
N-acetyltransferase 8 (GCN5-related, putative)
NCBI Official Symbol
NAT8
NCBI Official Synonym Symbols
GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
NCBI Protein Information
N-acetyltransferase 8
UniProt Protein Name
N-acetyltransferase 8
Protein Family
UniProt Gene Name
NAT8
UniProt Synonym Gene Names
ATase2; CCNAT
UniProt Entry Name
NAT8_HUMAN

NCBI Description

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

NAT8: Plays a role in regulation of gastrulation. Belongs to the camello family.

Protein type: Cell adhesion; EC 2.3.1.80; Membrane protein, multi-pass; Membrane protein, integral; Acetyltransferase

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; ER-Golgi intermediate compartment; integral to membrane

Molecular Function: lysine N-acetyltransferase activity; cysteine-S-conjugate N-acetyltransferase activity; protein binding

Biological Process: response to drug; glutathione metabolic process; beta-amyloid metabolic process; peptidyl-lysine N6-acetylation; negative regulation of apoptosis

Research Articles on NAT8

Similar Products

Product Notes

The NAT8 nat8 (Catalog #AAA9128768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAT8 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NAT8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the NAT8 nat8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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