Rabbit anti-Human NAT8 Polyclonal Antibody | anti-NAT8 antibody

NAT8, ID (NAT8, CML1, GLA, TSC501, Probable N-acetyltransferase 8, Camello-like protein 1) (AP)

Cat. Num. AAA6323957

• Gene Names: NAT8; GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NAT8; Polyclonal Antibody; ID (NAT8; CML1; GLA; TSC501; Probable N-acetyltransferase 8; Camello-like protein 1) (AP); Camello-like protein 1; anti-NAT8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NAT8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NAT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 120-150 from the Central region of human NAT8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NAT8 Antibody (Center) in ZR-75-1 cell line lysates (35ug/lane). NAT8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(NAT8 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NAT8 antibody

This protein, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

Product Categories/Family for anti-NAT8 antibody

Antibodies; Enzymes; Acetyltransferase

References

Ko, M.H., et al. J. Biol. Chem. 284(4):2482-2492(2009). Juhanson, P., et al. BMC Med. Genet. 9, 25 (2008). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 117190517
• NCBI GeneID: 9027
• NCBI Accession #: NP_003951.3
• NCBI GenBank Nucleotide #: NM_003960.3
• UniProt Accession #: Q9UHE5; O75839; Q6LEU4; Q96QI8; Q9UQ17
• Molecular Weight: 25,619 Da
• NCBI Official Full Name: N-acetyltransferase 8
• NCBI Official Synonym Full Names: N-acetyltransferase 8 (putative)
• NCBI Official Symbol: NAT8
• NCBI Official Synonym Symbols: GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
• NCBI Protein Information: N-acetyltransferase 8
• UniProt Protein Name: N-acetyltransferase 8
• Protein Family: N-acetyltransferase
• UniProt Gene Name: NAT8
• UniProt Synonym Gene Names: ATase2; CCNAT

NCBI Description

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

NAT8: Plays a role in regulation of gastrulation. Belongs to the camello family.

Protein type: Acetyltransferase; Cell adhesion; EC 2.3.1.80; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; endoplasmic reticulum-Golgi intermediate compartment; endoplasmic reticulum-Golgi intermediate compartment membrane; integral component of membrane

Molecular Function: cysteine-S-conjugate N-acetyltransferase activity; lysine N-acetyltransferase activity; protein binding

Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; glutathione metabolic process; negative regulation of apoptosis; peptidyl-lysine N6-acetylation; response to drug