Rabbit anti-Human NAT5 Polyclonal Antibody | anti-NAT5 antibody

NAT5, CT (NAA20, NAT5, N-alpha-acetyltransferase 20, N-acetyltransferase 5, N-terminal acetyltransferase B complex catalytic subunit NAA20, N-terminal acetyltransferase B complex catalytic subunit NAT5, NatB catalytic subunit) (PE)

Cat. Num. AAA6323945

• Gene Names: NAA20; NAT3; NAT5; dJ1002M8.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NAT5; Polyclonal Antibody; CT (NAA20; N-alpha-acetyltransferase 20; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5; NatB catalytic subunit) (PE); NAA20; NatB catalytic subunit; anti-NAT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NAT5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NAT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the C-terminal region of human NAT5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NAT5 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NAT5 antibody detected the NAT5 protein (arrow).)

Related Product Information for anti-NAT5 antibody

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM].

Product Categories/Family for anti-NAT5 antibody

Antibodies; Enzymes; Acetyltransferase

References

Kuo, H.P., et al. Sci Signal 3 (108), RA9 (2010): Evjenth, R., et al. J. Biol. Chem. 284(45):31122-31129(2009). Polevoda, B., et al. BMC Proc 3 SUPPL 6, S2 (2009): Starheim, K.K., et al. Biochem. J. 415(2):325-331(2008). Sanchez-Puig, N., et al. Protein Sci. 15(8):1968-1976(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 31563514
• NCBI GeneID: 51126
• NCBI Accession #: NP_852669.1; NP_852668.1; NP_057184.1
• NCBI GenBank Nucleotide #: NM_181528.3
• UniProt Accession #: P61599; Q5TFT7; Q9D7H8; Q9H0Y4; Q9NQH6; Q9Y6D2; A6NHA3; B2R4G4
• Molecular Weight: 20,368 Da
• NCBI Official Full Name: N-alpha-acetyltransferase 20 isoform c
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 20, NatB catalytic subunit
• NCBI Official Symbol: NAA20
• NCBI Official Synonym Symbols: NAT3; NAT5; dJ1002M8.1
• NCBI Protein Information: N-alpha-acetyltransferase 20; natB catalytic subunit; natB complex subunit NAT5; N-acetyltransferase 3 homolog; methionine N-acetyltransferase; N-acetyltransferase 5 (GCN5-related, putative); N-terminal acetyltransferase complex ARD1 subunit; N-acetyltran
• UniProt Protein Name: N-alpha-acetyltransferase 20
• Protein Family: Nucleobase-ascorbate transporter
• UniProt Gene Name: NAA20
• UniProt Synonym Gene Names: NAT5; NatB complex subunit NAT5
• UniProt Entry Name: NAA20_HUMAN

NCBI Description

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]

Uniprot Description

NAA20: Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. Belongs to the acetyltransferase family. ARD1 subfamily.

Protein type: Amino Acid Metabolism - phenylalanine; Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.88; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: peptide alpha-N-acetyltransferase activity

Biological Process: N-terminal peptidyl-methionine acetylation