Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat NAT10/hALP Polyclonal Antibody | anti-NAT10 antibody
Goat anti-NAT10/hALP Antibody
Gene Names
NAT10; ALP; NET43
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NAT10/hALP; Polyclonal Antibody; Goat anti-NAT10/hALP Antibody; NAT10; N-acetyltransferase 10; ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; KIAA1709; hALP; N-acetyltransferase-like; N-acetyltransferase-like protein; NAT10 antibody; N-acetyltransferase 10 antibody; ALP antibody; DKFZp434C116 antibody; FLJ10774 antibody; FLJ12179 antibody; FLJ23850 antibody; KIAA1709 antibody; hALP antibody; N-acetyltransferase-like antibody; N-acetyltransferase-like protein antibody; NAT10 / hALP; anti-NAT10 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KSDKKRKLEAKQ
Sequence Length
1025
Applicable Applications for anti-NAT10 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-KSDKKRKLEAKQ, from the internal region (near the C Terminus) of the protein sequence according to NP_078938.1.
Epitope
Internal region (near the C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,297 Da
NCBI Official Full Name
N-acetyltransferase 10 (GCN5-related)
NCBI Official Synonym Full Names
N-acetyltransferase 10
NCBI Official Symbol
NAT10
NCBI Official Synonym Symbols
ALP; NET43
NCBI Protein Information
RNA cytidine acetyltransferase
UniProt Protein Name
RNA cytidine acetyltransferase
Protein Family
UniProt Gene Name
NAT10
UniProt Entry Name
NAT10_HUMAN
Uniprot Description
ALP: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division. Belongs to the UPF0202 family.
Protein type: Acetyltransferase; Nucleolus; EC 2.3.1.-
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: membrane; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; nucleus; telomerase holoenzyme complex
Molecular Function: ATP binding; N-acetyltransferase activity; protein binding
Biological Process: negative regulation of telomere maintenance via telomerase; rRNA modification; tRNA processing
Research Articles on NAT10
Similar Products
Product Notes
The NAT10 nat10 (Catalog #AAA420570) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NAT10/hALP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NAT10/hALP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the NAT10 nat10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KSDKKRKLEA KQ. It is sometimes possible for the material contained within the vial of "NAT10/hALP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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