Rabbit anti-Human NANS Polyclonal Antibody | anti-NANS antibody

Anti-NANS Antibody, Rabbit Polyclonal

Cat. Num. AAA8108592

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NANS; Polyclonal Antibody; Anti-NANS Antibody; Rabbit Polyclonal; NANS Antibody; Rabbit PAb; Antigen Affinity Purified; N-acetylneuraminic acid synthase; Anti-HEL-S-100 Antibody; Anti-SAS Antibody; Anti-SEMDCG Antibody; Anti-SEMDG Antibody; anti-NANS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NANS
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NANS antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human NANS fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NANS fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NANS rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:40 kDa)

Related Product Information for anti-NANS antibody

NANS (N-Acetylneuraminate Synthase) is a Protein Coding gene. The NANS gene, located on 9q22.33, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. Diseases associated with NANS include Spondyloepimetaphyseal Dysplasia, Genevieve Type, and Cleft Palate, Isolated. Among its related pathways are Transport to the Golgi and subsequent modification and Metabolism of proteins.

References

van Karnebeek CD, et al. (2016) Nans-mediated synthesis of sialic acid is required for brain and skeletal development. Nat Genet 48 (7): 777-784.Lawrence SM, et al. (2000) Cloning and expression of the human n-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-d-glycero-d-galacto-nononic acid biosynthetic ability. J Biol Chem 275 (23): 17869-17877.Hamada T, et al. (2006) Solution structure of the antifreeze-like domain of human sialic acid synthase. Protein Sci 15 (5): 1010-1016.