Rabbit anti-Human NALP7 Polyclonal Antibody | anti-NLRP7 antibody

NALP7 Antibody

Cat. Num. AAA150857

• Gene Names: NLRP7; HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NALP7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NALP7; Polyclonal Antibody; NALP7 Antibody; HYDM; PAN7; NOD12; PYPAF3; CLR19.4; NACHT; LRR and PYD domains-containing protein 7; Nucleotide-binding oligomerization domain protein 12; NLR family; pyrin domain containing 7; anti-NLRP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NALP7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1009

• Applicable Applications for anti-NLRP7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NALP7 antibody can be used for detection of NALP7 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NALP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP7.
• Buffer: NALP7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NALP7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NALP7 in K562 cell lysate with NALP7 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NALP7 in human brain tissue with NALP7 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NALP7 in human brain tissue with NALP7 antibody at 20 μg/mL.)

Related Product Information for anti-NLRP7 antibody

NALP7 Antibody: NOD proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins. NALP7, also known as PYPAF3, a member of the related PYRIN-containing APAF1-like proteins (PYPAFs/NALPs) is thought to play a crucial role in cell proliferation. NALP7 has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. It is expressed in numerous tissues including uterus and ovary, with low levels in heart and brain. NALP7 inhibits caspase-1-dependent interleukin-1β secretion and is a feedback regulator of interleukin-1β secretion. Defects in the NALP7 gene are known to cause the formation of a hydatidiform mole (HYDM) and reduce the growth of carcinoma cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 46049100
• NCBI GeneID: 199713
• NCBI Accession #: NP_631915
• NCBI GenBank Nucleotide #: NM_139176.3
• UniProt Accession #: Q8WX94; Q32MH8; Q7RTR1; E9PE16
• Molecular Weight: 118,303 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 7
• NCBI Official Symbol: NLRP7
• NCBI Official Synonym Symbols: HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 7; PYRIN-containing Apaf1-like protein 3; NACHT, LRR and PYD containing protein 7; NACHT, leucine rich repeat and PYD containing 7; nucleotide-binding oligomerization domain protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 7
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 7
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP7
• UniProt Synonym Gene Names: NALP7; NOD12; PYPAF3
• UniProt Entry Name: NALP7_HUMAN

NCBI Description

This gene encodes a member of the NACHT, leucine rich repeat, and PYD containing (NLRP) protein family. It has an N-terminal pyrin domain, followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. NLRP proteins are implicated in the activation of proinflammatory caspases through multiprotein complexes called inflammasomes. This gene may act as a feedback regulator of caspase-1-dependent interleukin 1-beta secretion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NLRP7: Inhibits CASP1/caspase-1-dependent IL1B secretion. Defects in NLRP7 are the cause of hydatidiform mole recurrent type 1 (HYDM1). A disorder characterized by excessive trophoblast development and the presence of a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the NLRP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 19q13.42

Molecular Function: ATP binding

Disease: Hydatidiform Mole, Recurrent, 1