Western Blot (WB)
(Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide. )
Rabbit anti-Human, Mouse NADE Polyclonal Antibody | anti-NGFRAP1 antibody
NADE Antibody
Gene Names
NGFRAP1; Bex; BEX3; NADE; HGR74; DXS6984E
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
NADE Antibody is Ion exchange chromatography purified.
Synonyms
NADE; Polyclonal Antibody; NADE Antibody; Bex; BEX3; HGR74; DXS6984E; Protein BEX3; Brain-expressed X-linked protein 3; nerve growth factor receptor (TNFRSF16) associated protein 1; anti-NGFRAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
NADE Antibody is Ion exchange chromatography purified.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
111
Applicable Applications for anti-NGFRAP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
NADE antibody can be used for detection of NADE by Western blot at 1 mug/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 mug/mL.
Conjugate
Unconjugated
Immunogen
NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE.
Buffer
NADE Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
NADE antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide. )
Western Blot (WB)
(Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide. )
Immunohistochemistry (IHC)
(Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.)
Related Product Information for anti-NGFRAP1 antibody
NADE Antibody: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,762 Da
NCBI Official Full Name
protein BEX3 isoform b
NCBI Official Synonym Full Names
nerve growth factor receptor (TNFRSF16) associated protein 1
NCBI Official Symbol
NGFRAP1
NCBI Official Synonym Symbols
Bex; BEX3; NADE; HGR74; DXS6984E
NCBI Protein Information
protein BEX3; brain-expressed X-linked protein 3; p75NTR-associated cell death executor; ovarian granulosa cell 13.0 kDa protein HGR74
UniProt Protein Name
Protein BEX3
UniProt Gene Name
NGFRAP1
UniProt Synonym Gene Names
BEX3; DXS6984E; NADE
UniProt Entry Name
BEX3_HUMAN
Uniprot Description
NGFRAP1: May be a signaling adapter molecule involved in p75NTR- mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death. May play an important role in the pathogenesis of neurogenetic diseases. Belongs to the BEX family.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: Xq22.2
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: protein binding; metal ion binding; death receptor binding; caspase activator activity
Biological Process: caspase activation; nerve growth factor receptor signaling pathway; induction of apoptosis via death domain receptors; multicellular organismal development; regulation of caspase activity
Research Articles on NGFRAP1
Similar Products
Product Notes
The NGFRAP1 ngfrap1 (Catalog #AAA150522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NADE Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NADE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). NADE antibody can be used for detection of NADE by Western blot at 1 mug/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. Researchers should empirically determine the suitability of the NGFRAP1 ngfrap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NADE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
