Monkey Plakophilin 1 ELISA Kit | PKP1 elisa kit

Monkey Plakophilin 1 ELISA Kit

Cat. Num. AAA734931

• Gene Names: PKP1; B6P
• Reactivity: Monkey
• Synonyms: Plakophilin 1; Monkey Plakophilin 1 ELISA Kit; PKP1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PKP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PKP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKP1 elisa kit

Principle of the Assay: PKP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKP1 antibody and an PKP1-HRP conjugate. The assay sample and buffer are incubated together with PKP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKP1 concentration since PKP1 from samples and PKP1-HRP conjugate compete for the anti-PKP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PKP1 from the sample, fewer sites are left to bind PKP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKP1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53729346
• NCBI GeneID: 5317
• NCBI Accession #: NP_000290.2
• NCBI GenBank Nucleotide #: NM_000299.3
• Molecular Weight: 80,497 Da
• NCBI Official Full Name: plakophilin-1 isoform 1b
• NCBI Official Synonym Full Names: plakophilin 1
• NCBI Official Symbol: PKP1
• NCBI Official Synonym Symbols: B6P
• NCBI Protein Information: plakophilin-1; band 6 protein
• UniProt Protein Name: Plakophilin-1
• Protein Family: Plakophilin
• UniProt Gene Name: PKP1
• UniProt Synonym Gene Names: B6P
• UniProt Entry Name: PKP1_HUMAN

NCBI Description

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

plakophilin 1: Seems to play a role in junctional plaques. Contributes to epidermal morphogenesis. Defects in PKP1 are the cause of ectodermal dysplasia- skin fragility syndrome (EDSFS); also known as McGrath syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSFS is characterized by features of both cutaneous fragility and congenital ectodermal dysplasia affecting abnormalities in other epithelia or tissues. Desmosomes in the skin are small and poorly formed with widening of keratinocyte intercellular spaces and perturbed desmosome/keratin intermediate filament interactions. Belongs to the beta-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; desmosome; intracellular membrane-bound organelle; plasma membrane; intermediate filament; nucleus

Molecular Function: signal transducer activity; protein binding; intermediate filament binding; structural constituent of epidermis; lamin binding

Biological Process: cell-cell adhesion; apoptosis; multicellular organismal development; intermediate filament bundle assembly; cell adhesion; signal transduction; cell structure disassembly during apoptosis

Disease: Ectodermal Dysplasia/skin Fragility Syndrome