Rabbit N-Cadherin Polyclonal Antibody | anti-CDH2 antibody

N-Cadherin (a.a. 811-824)

Cat. Num. AAA472034

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: N-Cadherin; Polyclonal Antibody; N-Cadherin (a.a. 811-824); Cadherin-2; Neural-Cadherin; CD325 antigen; anti-CDH2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using N-cadherin (a.a. 811-824) peptide. The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with Ecadherin.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CDH2 antibody: ELISA, Western Blot, Immunoprecipitation
• Application Notes: ELISA: 1:2000; Immunoprecipitation: 1:100; Western Blot: 1:1000
• Immunogen: unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids 811 to 824 from human Ncadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in Rcadherin.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-CDH2 antibody

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

References

• Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463
• Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
• Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

NCBI and Uniprot Product Information

• NCBI GI #: 253483
• NCBI GeneID: 1000
• UniProt Accession #: P19022; Q14923; Q8N173; B0YIY6
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: N-cadherin
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; OTTHUMP00000066304; OTTHUMP00000067378; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD
• UniProt Entry Name: CADH2_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Uniprot Description

Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

Subunit structure: Interacts with CDCP1. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway

By similarity. Ref.10

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 5 cadherin domains.