Immunohistochemistry (IHC)
(Immunochemical staining of human MZT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human MZT1 Polyclonal Antibody | anti-MZT1 antibody
Anti-MZT1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
MZT1; Polyclonal Antibody; Anti-MZT1 Antibody; Rabbit Polyclonal; MZT1 Antibody; Rabbit PAb; Antigen Affinity Purified; mitotic spindle organizing protein 1; Anti-C13orf37 Antibody; Anti-MOZART1 Antibody; anti-MZT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MZT1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MZT1 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human MZT1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MZT1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human MZT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human MZT1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-MZT1 antibody
Mzt1 is small (~8.5 kDa), binds directly to core gamma-TuRC components, and appears to mediate the interaction between gamma-TuRCs and proteins that tether gamma-TuRCs to MTOCs. MZT1 knockdown causes severe cellular defects under conditions that leave the gamma-tubulin ring complex (gammaTuRC) intact, suggesting that the essential function of MZT1 is not in gammaTuRC assembly. Instead, MZT1 specifically binds fully assembled gammaTuRC to enable interaction with NEDD1 for targeting, and with the CM1 domain of CDK5RAP2 for stimulating nucleation activity. Thus, MZT1 is a 'priming factor' for gammaTuRC that allows spatial regulation of nucleation. MOZART1/Mzt1 is required for the localization of gamma-tubulin complexes to microtubule (MT)-organizing centers from yeast to human cells. Mzt1 is critical to stabilize Alp6, the S. pombe homolog of human gamma-TUSC protein GCP3, in an "interaction-competent" form within the gamma-TUSC.
References
Tovey CA, et al. (2018) -turc heterogeneity revealed by analysis of mozart1. Curr Biol 28 (14): 2314-2323.e2316.Leong SL, et al. (2019) Reconstitution of microtubule nucleation in vitro reveals novel roles for mzt1. Curr Biol 29 (13): 2199-2207.e2110.Cota RR, et al. (2017) Mzt1 regulates microtubule nucleation by linking gammaturc assembly to adapter-mediated targeting and activation. J Cell Sci 130 (2): 406-419.
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Product Notes
The MZT1 (Catalog #AAA8109142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MZT1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MZT1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the MZT1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MZT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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