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Western Blot (WB) (Western Blot analysis of 293T cells with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:2000)

Rabbit MYPT1 Polyclonal Antibody | anti-MYPT1 antibody

Phospho-MYPT1 (T853) Polyclonal Antibody

Gene Names
Ppp1r12a; Mypt1; AA792106; AV099298; D10Ertd625e; 1200015F06Rik; 5730577I22Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purified
Synonyms
MYPT1; Polyclonal Antibody; Phospho-MYPT1 (T853) Polyclonal Antibody; PPP1R12A; MBS; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; anti-MYPT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Concentration
1mg/ml (varies by lot)
Sequence Length
79
Applicable Applications for anti-MYPT1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000, IHC: 1:50-1:200, ELISA: 1:5000-1:20000
Immunogen
Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of 293T cells with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB) (Western Blot analysis of 293T cells with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:2000)

Immunofluorescence (IF)

(Immunofluorescence analysis of Human lung tissue with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:200)

Immunofluorescence (IF) (Immunofluorescence analysis of Human lung tissue with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:200)
Product Categories/Family for anti-MYPT1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 115 kDa
NCBI Official Full Name
MYPT1, partial
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory (inhibitor) subunit 12A
NCBI Official Symbol
Ppp1r12a
NCBI Official Synonym Symbols
Mypt1; AA792106; AV099298; D10Ertd625e; 1200015F06Rik; 5730577I22Rik
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Gene Name
Ppp1r12a
UniProt Synonym Gene Names
Mypt1; Myosin phosphatase target subunit 1
UniProt Entry Name
MYPT1_MOUSE

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytoplasm; focal adhesion; kinetochore; Z disc

Molecular Function: enzyme inhibitor activity; phosphatase regulator activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding

Biological Process: centrosome organization and biogenesis; mitosis; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; signal transduction

Research Articles on MYPT1

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Product Notes

The MYPT1 ppp1r12a (Catalog #AAA2539561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MYPT1 (T853) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000, IHC: 1:50-1:200, ELISA: 1:5000-1:20000. Researchers should empirically determine the suitability of the MYPT1 ppp1r12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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