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Western Blot (WB) (Western Blot analysis of Jurkat cells using MYPT1 Polyclonal Antibody at dilution of 1:2000.)

MYPT1 antibody kit

MYPT1 Antibody Sampler Kit

Gene Names
Ppp1r12a; Mypt1; AA792106; AV099298; D10Ertd625e; 1200015F06Rik; 5730577I22Rik
Applications
Western Blot
Synonyms
MYPT1; MYPT1 Antibody Sampler Kit; MYPT1 antibody kit
Ordering
For Research Use Only!
Sequence Length
79
Applicable Applications for MYPT1 antibody kit
Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Jurkat cells using MYPT1 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB) (Western Blot analysis of Jurkat cells using MYPT1 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of 293T cells with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB) (Western Blot analysis of 293T cells with Phospho-MYPT1 (Thr853) Polyclonal Antibody at dilution of 1:2000)
Related Product Information for MYPT1 antibody kit
Background: Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist. The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cdelta alters the conformation of the catalytic cleft and increases enzyme activity and specificity. Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain. Related family members include MBS85, MYPT3, and TIMAP. Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho- associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
111,809 Da
NCBI Official Full Name
MYPT1, partial
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory (inhibitor) subunit 12A
NCBI Official Symbol
Ppp1r12a
NCBI Official Synonym Symbols
Mypt1; AA792106; AV099298; D10Ertd625e; 1200015F06Rik; 5730577I22Rik
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Gene Name
Ppp1r12a
UniProt Synonym Gene Names
Myosin phosphatase target subunit 1

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 10 D1|10 56.33 cM

Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytoplasm; cytosol; focal adhesion; kinetochore; PTW/PP1 phosphatase complex; Z disc

Molecular Function: 14-3-3 protein binding; enzyme inhibitor activity; phosphatase regulator activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding

Biological Process: centrosome cycle; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; signal transduction

Research Articles on MYPT1

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Product Notes

The MYPT1 ppp1r12a (Catalog #AAA2550063) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MYPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the MYPT1 ppp1r12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYPT1, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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