Rabbit anti-Human, Mouse Myotubularin Related Protein 5 Polyclonal Antibody | anti-SBF1 antibody

Myotubularin Related Protein 5, CT (MTMR5, DKFZp761D0422, MGC99700, SET Binding Factor 1, SBF1)

Cat. Num. AAA627420

• Gene Names: SBF1; MTMR5; MGC99700; DKFZp761D0422
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Myotubularin Related Protein 5; Polyclonal Antibody; CT (MTMR5; DKFZp761D0422; MGC99700; SET Binding Factor 1; SBF1); Anti -Myotubularin Related Protein 5; anti-SBF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MTMR5. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-SBF1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human MTMR5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SBF1 antibody

The myotubularin (MTM) family constitutes one of the largest and most highly conserved protein-tyrosine phosphatase (PTP) subfamilies. Myotubularins, contain the consensus active site of tyrosine phosphatases but otherwise shows no homology to other phosphatases. PTP's usually act on substrates containing only phosphotyrosine sites, but myotubularins were shown to act on both phosphotyrosine and phosphoserine (dual specific). The enzymatic activity of myotubularins had not been demonstrated previously because it lacks catalytically active residues in tyrosine phosphatase/dual-specific phosphatase active site. The active site is however sufficiently preserved to bind phosphorylated substrates, and may protect from phosphatases. It was reported that interaction of myotubularin family members makes one of them catalytically active. MTMR5 can interact with MTMR2 and regulates the enzymatic activity and the subcellular localization of MTMR2. MTMR5 inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.Other suggested roles for MTMR5 are in spermatogenesis and germ cell differentiation. Binding partners for MTMR5 include the SET domain of MLL/HRX and SUV39H1.

Product Categories/Family for anti-SBF1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 239735519
• NCBI GeneID: 6305
• NCBI Accession #: NP_002963.2
• NCBI GenBank Nucleotide #: NM_002972.2
• UniProt Accession #: O95248; O60228; Q5JXD8; Q5PPM2; Q96GR9; Q9UGB8; A6PVG9; Q59EX9; Q68CX1; Q6PGN5; Q86TK5
• Molecular Weight: 208,315 Da
• NCBI Official Full Name: myotubularin-related protein 5
• NCBI Official Synonym Full Names: SET binding factor 1
• NCBI Official Symbol: SBF1
• NCBI Official Synonym Symbols: MTMR5; MGC99700; DKFZp761D0422
• NCBI Protein Information: myotubularin-related protein 5; OTTHUMP00000196597; OTTHUMP00000196599; SET-binding factor 1; myotubularin related 5
• UniProt Protein Name: Myotubularin-related protein 5
• UniProt Gene Name: SBF1
• UniProt Synonym Gene Names: MTMR5
• UniProt Entry Name: MTMR5_HUMAN

NCBI Description

This gene encodes a member of the protein-tyrosine phosphatase family. However, the encoded protein does not appear to be a catalytically active phosphatase because it lacks several amino acids in the catalytic pocket. This protein contains a Guanine nucleotide exchange factor (GEF) domain which is necessary for its role in growth and differentiation. Mutations in this gene have been associated with Charcot-Marie-Tooth disease 4B3. Pseudogenes of this gene have been defined on chromosomes 1 and 8. [provided by RefSeq, Dec 2014]

Uniprot Description

MTMR5: a pseudophosphatase and nuclear guanine nucleotide exchange factor (GEF) that activates RAB28, a small G protein of the Rab subfamily. Lacks several amino acids in the catalytic pocket, which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. Defects in MTMR5 are the cause of Charcot-Marie-Tooth disease type 4B3 (CMT4B3). Charcot-Marie-Tooth disease is the most common inherited disorder of the peripheral nervous system. Interacts with the SET domain of MLL1. Interacts with SUV39H1. Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GEFs, Rab; GEFs; Inhibitor

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: integral to membrane; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; Rab guanyl-nucleotide exchange factor activity

Biological Process: spermatogenesis; protein amino acid dephosphorylation

Disease: Charcot-marie-tooth Disease, Type 4b3