Rabbit anti-Human MYOT Polyclonal Antibody | anti-MYOT antibody

MYOT, ID (MYOT, TTID, Myotilin, 57kD cytoskeletal protein, Myofibrillar titin-like Ig domains protein, Titin immunoglobulin domain protein)

Cat. Num. AAA649349

• Gene Names: MYOT; MFM3; TTID; TTOD; LGMD1; LGMD1A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MYOT; Polyclonal Antibody; ID (MYOT; TTID; Myotilin; 57kD cytoskeletal protein; Myofibrillar titin-like Ig domains protein; Titin immunoglobulin domain protein); Anti -MYOT; anti-MYOT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MYOT antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: MYOT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human MYOT.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYOT Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MYOT antibody detected the MYOT protein (arrow).)

Related Product Information for anti-MYOT antibody

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Product Categories/Family for anti-MYOT antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 209693444
• NCBI GeneID: 9499
• NCBI Accession #: NP_001129412.1
• NCBI GenBank Nucleotide #: NM_001135940.1
• UniProt Accession #: Q9UBF9; A0A4R6; B4DT79
• Molecular Weight: 55,395 Da
• NCBI Official Full Name: myotilin isoform b
• NCBI Official Synonym Full Names: myotilin
• NCBI Official Symbol: MYOT
• NCBI Official Synonym Symbols: MFM3; TTID; TTOD; LGMD1; LGMD1A
• NCBI Protein Information: myotilin; 57 kDa cytoskeletal protein; myofibrillar titin-like Ig domains protein; titin immunoglobulin domain protein (myotilin)
• UniProt Protein Name: Myotilin
• Protein Family: Myotilin
• UniProt Gene Name: MYOT
• UniProt Synonym Gene Names: TTID
• UniProt Entry Name: MYOTI_HUMAN

NCBI Description

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008]

Uniprot Description

Function: Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells. Ref.7

Subunit structure: Homodimer. Interacts with ACTA1, ACTN1, FLNA, FLNB, FLNC and MYOZ2. Interacts with the C-terminal region of MYOZ1. Ref.2 Ref.6 Ref.7 Ref.8 Ref.11

Subcellular location: Cell membrane › sarcolemma. Cytoplasm › cytoskeleton. Cytoplasm › myofibril › sarcomere › Z line. Note: Sarcomeric, also localized to the sarcolemma. Colocalizes with MYOZ1 at the Z-lines in skeletal muscle. Ref.2 Ref.8

Tissue specificity: Expressed in skeletal muscle (at protein level). Expressed in skeletal muscle, heart, bone marrow and thyroid gland. Ref.1 Ref.2

Involvement in disease: Limb-girdle muscular dystrophy 1A (LGMD1A) [MIM:159000]: An autosomal dominant degenerative myopathy with onset within a mean age of 28 years. Characterized by progressive skeletal muscle weakness of the hip and shoulder girdles, later progressing to include distal weakness, as well as a distinctive dysarthric pattern of speech. Affected muscle exhibits disorganization and streaming of the Z-line.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12Myopathy, myofibrillar, 3 (MFM3) [MIM:609200]: A neuromuscular disorder characterized by progressive skeletal muscle weakness greater distally than proximally, tight heel cords, hyporeflexia, cardiomyopathy and peripheral neuropathy in some patients. Affected muscle exhibits disorganization and streaming of the Z-line, presence of large hyaline structures, excessive accumulation of myotilin and other ectopically expressed proteins and prominent congophilic deposits.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13Spheroid body myopathy (SBM) [MIM:182920]: Autosomal dominant form of myofibrillar myopathy (MFM), characterized by slowly progressing proximal muscle weakness and dysarthric nasal speech. There is no evidence of cardiomyopathy. Muscle biopsy shows spheroid bodies within the type I muscle fibers.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.15

Sequence similarities: Belongs to the myotilin/palladin family.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.