CD45/PTPRC recombinant protein

Recombinant Mouse CD45/PTPRC (C-6His)

Cat. Num. AAA2569372

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: CD45/PTPRC; Recombinant Mouse CD45/PTPRC (C-6His); B220; CD45 antigen; CD45; CD45R; LCA; L-CA; LY5; protein tyrosine phosphatase; receptor type; C; PTPRC; receptor-type tyrosine-protein phosphatase C; CD45/PTPRC recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Gln26--Lys566

• Species: Mouse
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Receptor-type Tyrosine-protein Phosphatase C is produced by our Mammalian expression system and the target gene encoding Gln26-Lys566 is expressed with a 6His tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for CD45/PTPRC recombinant protein

Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.

NCBI and Uniprot Product Information

• UniProt Accession #: P06800
• Molecular Weight: Mol. Mass: 59.5kDa; AP Mol. Mass: 120-160kDa
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: Ly-5
• UniProt Entry Name: PTPRC_MOUSE