Rabbit anti-Human MYOM1 Polyclonal Antibody | anti-MYOM1 antibody

MYOM1, ID (MYOM1, Myomesin-1, 190kD connectin-associated protein, 190kD titin-associated protein, Myomesin family member 1) (AP)

Cat. Num. AAA6323517

• Gene Names: MYOM1; SKELEMIN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYOM1; Polyclonal Antibody; ID (MYOM1; Myomesin-1; 190kD connectin-associated protein; 190kD titin-associated protein; Myomesin family member 1) (AP); Myomesin family member 1; anti-MYOM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MYOM1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYOM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 903-933 amino acids from the Central region of human MYOM1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MYOM1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MYOM1 antibody detected the MYOM1 protein (arrow).)

Immunohistochemistry (IHC)

(MYOM1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYOM1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MYOM1 antibody

The giant protein titin, together with its associated proteins, interconnects the major structure of sarcomeres, the M bands and Z discs. The C-terminal end of the titin string extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD (myomesin 1) and 165 kD (myomesin 2). This protein, myomesin 1, like myomesin 2, titin, and other myofibrillar proteins contains structural modules with strong homology to either fibronectin type III (motif I) or immunoglobulin C2 (motif II) domains. Myomesin 1 and myomesin 2 each have a unique N-terminal region followed by 12 modules of motif I or motif II, in the arrangement II-II-I-I-I-I-I-II-II-II-II-II. The two proteins share 50% sequence identity in this repeat-containing region. The head structure formed by these 2 proteins on one end of the titin string extends into the center of the M band. The integrating structure of the sarcomere arises from muscle-specific members of the superfamily of immunoglobulin-like proteins. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-MYOM1 antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Schoenauer, R., et al. J. Mol. Biol. 349(2):367-379(2005). Hornemann, T., et al. J. Mol. Biol. 332(4):877-887(2003). Porter, J.D., et al. J. Exp. Biol. 206 (PT 17), 3101-3112 (2003): Agarkova, I., et al. J. Biol. Chem. 275(14):10256-10264(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 140560919
• NCBI GeneID: 8736
• NCBI Accession #: NP_003794.3; NP_062830.1
• NCBI GenBank Nucleotide #: NM_003803.3
• UniProt Accession #: P52179; Q14BD6; Q6H969; Q6ZUU0
• Molecular Weight: 187,627 Da
• NCBI Official Full Name: myomesin-1 isoform a
• NCBI Official Synonym Full Names: myomesin 1
• NCBI Official Symbol: MYOM1
• NCBI Official Synonym Symbols: SKELEMIN
• NCBI Protein Information: myomesin-1; EH-myomesin; myomesin 1, 185kDa; myomesin family member 1; myomesin 1 (skelemin) 185kDa; myomesin (M-protein) 1 (190kD); 190 kDa titin-associated protein; 190 kDa connectin-associated protein
• UniProt Protein Name: Myomesin-1
• Protein Family: Myomesin
• UniProt Gene Name: MYOM1
• UniProt Entry Name: MYOM1_HUMAN

NCBI Description

The giant protein titin, together with its associated proteins, interconnects the major structure of sarcomeres, the M bands and Z discs. The C-terminal end of the titin string extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD (myomesin 1) and 165 kD (myomesin 2). This protein, myomesin 1, like myomesin 2, titin, and other myofibrillar proteins contains structural modules with strong homology to either fibronectin type III (motif I) or immunoglobulin C2 (motif II) domains. Myomesin 1 and myomesin 2 each have a unique N-terminal region followed by 12 modules of motif I or motif II, in the arrangement II-II-I-I-I-I-I-II-II-II-II-II. The two proteins share 50% sequence identity in this repeat-containing region. The head structure formed by these 2 proteins on one end of the titin string extends into the center of the M band. The integrating structure of the sarcomere arises from muscle-specific members of the superfamily of immunoglobulin-like proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

myomesin 1: Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: striated muscle thick filament; M band

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; structural constituent of muscle

Biological Process: muscle contraction