Western Blot (WB)
((2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Myogenin/MYF4 Polyclonal Antibody | anti-MYOG antibody
Goat anti-Myogenin/MYF4 Antibody
Western Blot: Approx 38kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 25. 0kDa according to Human NP_002470.2 and of 25.2kDa according to Mouse NP_112466.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-4ug/ml.
Western Blot (WB)
((2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
Uniprot Description
myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 1q31-q41
Cellular Component: nucleoplasm; nucleus; transcription factor complex
Molecular Function: chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity
Biological Process: cell cycle; mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; myoblast differentiation; negative regulation of cell proliferation; negative regulation of glycolysis; ossification; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to gravity; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle atrophy; skeletal muscle development; skeletal muscle fiber development; skeletal muscle regeneration; striated muscle atrophy
Research Articles on MYOG
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Product Notes
The MYOG myog (Catalog #AAA422344) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Myogenin/MYF4 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Myogenin/MYF4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 38kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 25. 0kDa according to Human NP_002470.2 and of 25.2kDa according to Mouse NP_112466.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-4ug/ml. Researchers should empirically determine the suitability of the MYOG myog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TDAHNLHSLT SIVD. It is sometimes possible for the material contained within the vial of "Myogenin/MYF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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