Western Blot (WB)
(Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.)
Rabbit anti-Human MyoGEF Polyclonal Antibody | anti-MyoGEF antibody
MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6) (Biotin)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
PLEKHG6: Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: GEF; GEF, Rac/Rho
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: cleavage furrow; cytoplasm; microvillus; plasma membrane; spindle pole
Molecular Function: GTPase activator activity; protein binding; Rho guanyl-nucleotide exchange factor activity
Biological Process: positive regulation of GTPase activity; regulation of Rho protein signal transduction
Research Articles on MyoGEF
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Product Notes
The MyoGEF plekhg6 (Catalog #AAA6489014) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MyoGEF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MyoGEF plekhg6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MyoGEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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