Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | NGAL elisa kit

Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

Cat. Num. AAA2568041

• Reactivity: Human
• Synonyms: NGAL (Neutrophil Gelatinase Associated Lipocalin); Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit; NGAL; NGAL elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human NGAL in samples. No significant cross-reactivity or interference between Human NGAL and analogues was observed. This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma and other biological fluids.

• Assay Type: Sandwich ELISA
• Detection Method: Colormetric
• Tested Sample Types: Serum, plasma and other biological fluids
• Detection Range: 31.25~2000pg/mL
• Sensitivity: 18.75pg/ml
• Precision: Both intra-CV and inter-CV are < 10%.
• Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.
• Preparation and Storage: An unopened kit can be stored at 2-8 degree C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the *following conditions (manual) once the kit is received.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 49457137
• UniProt Accession #: P80188; P30150; Q5SYV9; Q5SYW0; Q6FGL5; Q92683; A6NII8; B4DWV4; B7ZAA2
• Molecular Weight: 22,588 Da
• NCBI Official Full Name: LCN2
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport