Rabbit anti-Human MYOD1 Polyclonal Antibody | anti-MYOD1 antibody

MYOD1 Polyclonal Antibody

Cat. Num. AAA127874

• Gene Names: MYOD1; PUM; MYF3; MYOD; bHLHc1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: MYOD1; Polyclonal Antibody; MYOD1 Polyclonal Antibody; MYF3; MYOD; PUM; bHLHc1; anti-MYOD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 320

• Applicable Applications for anti-MYOD1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Fusion protein of human MYOD1
• Calculated Molecular Weight: 35kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MYOD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-MYOD1 antibody

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1,2). This assay can be used to either identify multiple proteins associated with a specific region of the genome or to identify the many regions of the genome bound by a particular protein (3-6). ChIP can be used to determine the specific order of recruitment of various proteins to a gene promoter or to "measure" the relative amount of a particular histone modification across an entire gene locus (3,4). In addition to histone proteins, the ChIP assay can be used to analyze binding of transcription factors and co-factors, DNA replication factors, and DNA repair proteins. When performing the ChIP assay, cells are first fixed with formaldehyde, a reversible protein-DNA cross-linking agent that "preserves" the protein-DNA interactions occurring in the cell (1,2). Cells are lysed and chromatin is harvested and fragmented using either sonication or enzymatic digestion. Fragmented chromatin is then immunoprecipitated with antibodies specific to a particular protein or histone modification. Any DNA sequences that are associated with the protein or histone modification of interest will co-precipitate as part of the cross-linked chromatin complex and the relative amount of that DNA sequence will be enriched by the immunoselection process. After immunoprecipitation, the protein-DNA cross-links are reversed and the DNA is purified. Standard PCR or quantitative real-time PCR are often used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation (1,2). Alternatively, the ChIP assay can be combined with genomic tiling micro-array (ChIP on chip) techniques, high throughput sequencing (ChIP-Seq), or cloning strategies, all of which allow for genome-wide analysis of protein-DNA interactions and histone modifications (5-8).

Product Categories/Family for anti-MYOD1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 209572729
• NCBI GeneID: 4654
• UniProt Accession #: P15172; O75321
• Molecular Weight: 320
• NCBI Official Full Name: Myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: MYOD1
• NCBI Official Synonym Symbols: PUM; MYF3; MYOD; bHLHc1
• NCBI Protein Information: myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myoblast determination protein
• UniProt Gene Name: MYOD1
• UniProt Synonym Gene Names: BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
• UniProt Entry Name: MYOD1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: Transcription factor; DNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome