Rabbit anti-Human MyoD1 Polyclonal Antibody | anti-MyoD1 antibody

MyoD1, ID (MYOD1, BHLHC1, MYF3, MYOD, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3) (MaxLight 405)

Cat. Num. AAA6323478

• Gene Names: MYOD1; PUM; MYF3; MYOD; bHLHc1
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MyoD1; Polyclonal Antibody; ID (MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; Myogenic factor 3) (MaxLight 405); MYOD1; Myogenic factor 3; anti-MyoD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MyoD1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-MyoD1 antibody: Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 211-240 in the central region of human MyoD1, Swiss/UniProt Accession: P15172.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Hela cell line and human placenta tissue lysates (35ug/lane) usingMBS6011435. This demonstrates that MBS6011435 detected the MyoD1 protein (arrow).)

Testing Data

(All lanes: MBS6011435 (1:2000) Lane 1: Human skeletal muslce lysate Lane 2: HL-60 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: RD whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat at 1:10,000. Predicted band size: 35kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell usingMBS6011435. Hela cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with MBS6011435 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10mins). MyoD1 immunoreactivity is localized to nucleus significantly.)

Related Product Information for anti-MyoD1 antibody

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Product Categories/Family for anti-MyoD1 antibody

Antibodies; Transcription Factors

References

Xynos, A., et al. Stem Cells 28(5):965-973(2010). Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010). Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010). Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Yang, Z., et al. Genes Dev. 23(6):694-707(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23111009
• NCBI GeneID: 4654
• NCBI Accession #: NP_002469.2
• NCBI GenBank Nucleotide #: NM_002478.4
• UniProt Accession #: P15172; O75321
• Molecular Weight: 34,501 Da
• NCBI Official Full Name: myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: MYOD1
• NCBI Official Synonym Symbols: PUM; MYF3; MYOD; bHLHc1
• NCBI Protein Information: myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myoblast determination protein
• UniProt Gene Name: MYOD1
• UniProt Synonym Gene Names: BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
• UniProt Entry Name: MYOD1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; RNA splicing; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome